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. 2020 Apr 17;6:22. doi: 10.1038/s41420-020-0259-2

Fig. 5. PERK dampens ABT-263 killing activity.

Fig. 5

a Q-PCR analysis of PERK level in 92.1 uveal melanoma cells treated with control (siCtl) or PERK (siPERK) siRNA for 48 h. ***P-value < 0.001. b Western blot analysis to PERK and P-PERK in 92.1 cells treated with control (siCtl) or PERP (siPERK) siRNA. HSP90 is used as a loading control. c 92.1 uveal melanoma cells treated with control (siCtl) or PERK (siPERK) siRNA for 48 h before being exposed to ABT-263 3 μM for 48 h. Representative western blot to P-EIF2α, total EIF2α and PARP are shown. HSP90 serves as a loading control. d 92.1 cells were treated as in b. FACS analyses of Annexin V/DAPI double staining in uveal melanoma cells indicate alive (white) or dead (early, late apoptosis and necrosis in light grey) cells. ***P-value < 0.001. e 92.1 cells were treated as in b. FACS analyses of Annexin V/DAPI double staining in uveal melanoma cells indicate alive (white) or dead (early, late apoptosis and necrosis in light grey) cells. ***P-value < 0.001.