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. 2020 Apr 8;26:e923411-1–e923411-10. doi: 10.12659/MSM.923411

Figure 1.

Figure 1

JQ1 restrained the proliferation of B-ALL cell lines. (A) Drug sensitivity assay of NALM6, REH, SEM, and RS411 cell lines after treatment with gradient concentrations of JQ1 for 72 h. (B) The cell growth curve of NALM6, REH, SEM, and RS411 cells after treatment with DMSO or JQ1 (1 μM) for 5 days. (C) EdU- and PI-labeled cell cycle of NALM6, REH, SEM, and RS411 cells were analyzed after treatment with DMSO or JQ1 (1 μM) for 48 h. (D) Statistical analysis of cell cycle distributions in C. (E) Heatmap of cell cycle and apoptosis-related genes detected by RNA-seq in NALM6 cells after treatment with DMSO or JQ1 (1 μM) for 48 h. (F) Annexin V- and PI-labeled cell apoptosis of NALM6, REH, SEM, and RS411 cells analyzed by flow cytometry after DMSO or JQ1 (1 μM) treatment for 48 h. (G) Statistical analysis of cell apoptosis rates in F. (H) Expressions of cleaved caspase 9 and BCL-2 detected by Western blot in NALM6, REH, SEM, and RS411 cells after treatment with DMSO or JQ1 (1 μM) for 48 h.* P<0.05; ** P<0.01.