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. 2020 Apr 8;26:e923411-1–e923411-10. doi: 10.12659/MSM.923411

Figure 4.

Figure 4

JQ1 induced cell cycle arrest by inhibiting c-Myc-mediated glycolysis. (A) Protein expression of c-Myc determined by Western blot in NALM6, REH, SEM, and RS411 cells after treatment with DMSO or JQ1 (1 μM) (1, 2, and 4 μM) for 48 h. (B) Overexpression level of c-Myc in NALM6 and REH cells was checked by Western blot. (C, D) The expression level of c-Myc in NALM6 and REH cells tested by Western blot after treatment with DMSO or JQ1 (1 μM) in a time course. (E, F) EdU- and PI-labeled cell cycle of c-Myc-overexpressing NALM6 and REH cells analyzed by flow cytometry after DMSO or JQ1 (2 μM) treatment for 24 h. (G) Quantification of intracellular lactate in vector and c-Myc-overexpressing NALM6 cells after DMSO or JQ1 (2 μM) treatment for 24 h. (H) Quantification of intracellular ATP in vector and c-Myc-overexpressing NALM6 cells after DMSO or JQ1 (2 μM) treatment for 24 h. (I) Glucose uptake of vector and c-Myc-overexpressing NALM6 cells analyzed by flow cytometry after treatment with DMSO or JQ1 (2 μM) for 24 h. (J) Levels of HK2 and LDHA in c-Myc-overexpressing NALM6 and REH cells tested by Western blot after treatment with DMSO or JQ1 (2 μM) for 24 h.* P<0.05.