Table 3.

Guide RNA and detection PCR primer sequence for B7-H6 knockout with CRISPR-Cas9.

Guide RNA	Sequence
Control	5′-GTATTACTGATATTGGTGGG-3′
Cas1	5′-GGGTGACCACCACCTCACAT-3′
Cas2	5′-GAGCCATTGTGTCTCCATGG-3′
PCR primer	Sequence
Forward	5′-AACCCCTCAACATCACGTCT-3′
Reverse	5′-TGTTGCTAACCCCAACACCAT-3′