Fig. 4.

hISCs differentiate into osteogenic lineage and initiate adipogenic differentiation under inductive conditions. Phase-contrast microscopic view of hISCs and BM-MSCs cultured either in osteogenic differentiation medium (DIFF) (a) or in adipogenic differentiation medium (b). The deposition of calcified and mineralized ECM and the inclusion of lipid droplets were visualized by Alizarin Red and Oil Red O staining, respectively. Scale bar = 100 μm. mRNA expression of adipogenic genes (PPARγ, FABP4, and LPL) in hISCs and BM-MSCs was increased after 21 days in adipogenic differentiation medium (black column) as compared to day 0 (white column) (c). Values are normalized to RPS9 expression; n = 4. *p < 0.05, **p < 0.01 in differentiated versus undifferentiated cells