Fig. 3.

a Myofibre classification key for coverage of sarcolemmal protein markers (Dystrophin, α-sarcoglycan or β-dystroglycan). Sarcolemmal circumference coverage is used to place fibres in 4 categories. Protein negative (< 25% coverage) or varying degrees of protein positive (25–50%, 50–75% or 75–100% sarcolemmal circumference coverage). b Image panel highlighting dystrophin immunofluorescence staining (green 488) of CTRL_1, BMD_3 and DMD_3 and their corresponding dystrophin classification maps. Yellow regions show connective tissue or myofibres have not been recognised/detected during the fibre recognition phase