Fig. 3.

Pol θ generates locally templated insertions. (A) The difference in the fraction of repair products with noted size of insertion in POLQ-expressing cells vs. Polq^−/− was averaged across all five break sites. Insertions were defined as having 5 bp or more direct or inverse repeated sequence (DR, cyan; IR, blue) relative to flanking DNA (see also C). (B) The relative abundance of insertions with 5 bp or more repeated sequence is plotted according to the location of the repeat within flanking DNA for both DR (cyan) and IR (blue) insertions. (C) Model for generating direct repeat vs. inverted repeat templated insertions.