Fig. 4.

Impact of exercise on brain fatty acid profile. Frontal cortex and hippocampal samples were assayed for the profile of saturated (a) and unsaturated (b) fatty acids by metabolomics. c Immunoblotting was performed to assay the expression of the fatty acid synthase (FASN) protein in the running and inactive mice. Exercise upregulated FASN expression in the hippocampus but not in the frontal cortex. Upper graph, representative images, lower graph, quantitative data normalized to total protein level. Median with min. - max. *p < 0.05, **p < 0.01, *p < 0.05, **p < 0.01; n = 6–7