Fig. 3.
Transmissibility of I38-WT and 38T/F/M influenza A and B viruses in ferrets. Donor ferrets (n = 3, gold) were inoculated with 105 TCID50 units per ferret of recombinant influenza (A) A/CA/04 (H1N1)pdm09, (B), A/TX/71(H3N2), or (C) B/BR/60 (Victoria lineage) virus. Twenty-four hours later, DC cage mates (n = 3, blue) or AC cage mates (separated by a barrier that excluded physical contact, n = 3, red) were introduced. Virus titers (log10TCID50/mL; limit of detection 0.75-Log10TCID50/mL) were assayed in nasal washes collected at the indicated time points after donor inoculation. Each peak represents the shed virus titer of an individual animal. Donor B/BR/60 38F animals received 103.5 TCID50 units per ferret.