Table 1.
CB‐ELISA detection of mAb reactivity with TGEV strains
| mAb TGEV | TGEV strain | ||||
|---|---|---|---|---|---|
| Shizuoka | Purdue | Cz‐1970 | Cz‐1995 | V‐344 | |
| D7/G7 | |||||
| A | 0.737/0.094 | 0.659/0.035 | 0.606/0.027 | 0.701/0.021 | 0.507/0.026 |
| NA | 0.643 | 0.624 | 0.579 | 0.680 | 0.481 |
| %B | 87.2 | 94.7 | 95.5 | 97.0 | 94.9 |
| B7/F7* | |||||
| A | 1.004/0.397 | 1.168/0.563 | 0.829/0.292 | 1.051/0.210 | 1.281/0.773 |
| NA | 0.607 | 0.605 | 0.537 | 0.841 | 0.508 |
| %B | 60.5 | 51.8 | 64.8 | 80.0 | 39.7 |
| D3/G6 | |||||
| A | 0.023/0.006 | 0.033/0.011 | 0.017/0.002 | 0.483/0.011 | 0.542/0.028 |
| NA | 0.017 | 0.022 | 0.015 | 0.472 | 0.514 |
| %B | 73.9 | 66.7 | 88.2 | 97.7 | 94.8 |
| B4/F2 | |||||
| A | 0.128/0.034 | 0.141/0.045 | 0.106/0.021 | 0.649/0.013 | 0.596/0.047 |
| NA | 0.094 | 0.096 | 0.085 | 0.636 | 0.549 |
| %B | 73.4 | 68.1 | 80.2 | 98.0 | 92.1 |
| mAb mix | |||||
| A | 0.937/0.176 | 0.938/0.224 | 0.774/0.128 | 0.990/0.099 | 1.070/0.318 |
| NA | 0.761 | 0.714 | 0.646 | 0.891 | 0.752 |
| %B | 81.2 | 76.1 | 83.5 | 90.0 | 70.3 |
A, average absorbances obtained in wells containing SwSneg./pos; NA, net absorbance; %B, percentage of the reaction blocking in wells incubated with SwSpos; mAb mix, mixture of equal concentrations (w/v) of individual mAb.
Positive A, NA and %B values are given in bold.
The culture medium of the strain V‐344 was frozen 48 hpi (after development of CPE) and examined at working dilution 1 : 4. The remaining media were frozen 24 hpi (before CPE) and examined diluted 1 : 2.
*The culture medium of the strain V‐344 incubated with mAb B7/F7 was clearly positive when diluted 1 : 8 and 1 : 16 with the A, NA and %B values 0.992/0.379, 0.613 and 61.8%, and 0.415/0.095, 0.314 and 76.8% respectively.