Table 3.
Detection of TGEV in faecal samples obtained between 1 and 7 days after experimental infection. The influence of faecal sample dilutions on the CB‐ELISA results
| Faeces (dpi) | Faeces dilution 2× | Faeces dilution 10× | Faeces dilution 50× | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SwS neg. | SwS pos. | NA | %B | SwS neg. | SwS pos. | NA | %B | SwS neg. | SwS pos. | NA | %B | |
| 1 | 1.410 | 0.727 | 0.683 | 48.4 | 1.286 | 0.194 | 1.092 | 84.9 | 0.477 | 0.011 | 0.466 | 97.7 |
| 2 | 1.423 | 0.896 | 0.527 | 37.0 | 1.518 | 0.325 | 1.193 | 78.6 | 0.486 | 0.012 | 0.474 | 97.5 |
| 3 | 1.330 | 1.137 | 0.193 | 14.5 | 1.402 | 0.619 | 0.783 | 55.8 | 1.219 | 0.098 | 1.121 | 92.0 |
| 4 | 1.083 | 0.910 | 0.173 | 16.0 | 1.157 | 0.508 | 0.649 | 56.1 | 1.060 | 0.071 | 0.989 | 93.3 |
| 5 | 1.075 | 0.769 | 0.306 | 28.5 | 1.298 | 0.445 | 0.853 | 65.7 | 1.084 | 0.051 | 1.033 | 95.3 |
| 6 | 1.139 | 0.724 | 0.415 | 36.4 | 1.313 | 0.365 | 0.948 | 72.2 | 0.791 | 0.039 | 0.752 | 95.1 |
| 7 | 0.885 | 0.330 | 0.555 | 62.7 | 0.837 | 0.098 | 0.739 | 88.3 | 0.269 | 0.027 | 0.242 | 90.0 |
dpi, The day post‐infection.
CB‐ELISA examination was performed at various working dilutions of faecal samples (2×, 10×, 50×) and constant dilutions of SwSneg./pos. (40×), mAbTGEV mixture (10 000×) and HRPO‐SwAMoIg (10 000×).
Samples with positive NA (>0.1) and %B (>50.0) values are given in bold.