Figure 2.

Effect of GL on R5 HIV replication in MDMs transwell cultured with PMNs. MDMs (5 × 10⁵ cells ml⁻¹, lower chamber) were exposed to 1580 TCID₅₀ per ml of R5 HIV for 3 h. After washing, these cells were cultured with PMNs (5 × 10⁶ cells ml⁻¹, upper chamber) in double chamber transwells supplemented with or without GL (100 μg ml⁻¹). At 24 h after cultivation, the upper chamber was removed and cells in the lower chamber were cultured for an additional 14 days. As controls, with or without 100 μg ml⁻¹ of GL, MDMs exposed to the virus were cultured alone. The quantity of p24 antigen in the culture fluids of these cells was determined by ELISA. The results shown are representative of three replicate experiments. ^* P<0.01 compared with the untreated group.