Table 1.
Seropositivity for alpha and flavivirus antibodies in bats from Grenada
| Virus | Test | Species (# positive/# tested) Parishes Total % positive | Titres |
|---|---|---|---|
| CHIKV | PRNT50 |
Artibeus lituratus (1/2) St. George Artibeus jamaicensis (14/40) All parishes except St. Andrew 36% |
Positive at 1:10–>1:640 dilution |
| Bussuquara virus | PRNT80–90 |
A. jamaicensis (1/48) A. lituratus (0/2) St. David 2% |
Pos at PRNT80 (Neg at PRNT90) |
| DEN 1, 2, 3, 4 | PRNT80–90 | 0/0.0% | NA |
| WNV | PRNT80–90 | 0/0.0% | NA |
| SLEV | PRNT80–90 | 0/0.0% | NA |
| Rio Bravo virusa | PRNT80–90 | 0/0.0% | NA |
| VEEV | Epitope‐blocking ELISA |
A. jamaicensis (1/36) A. lituratus (0/2) St. John 2.6% |
30.38% inhibition |
| WEEV | Epitope‐blocking ELISA | 0/0.0% | NA |
| EEEV | Epitope‐blocking ELISA | 0/0.0% | NA |
CHIKV, chikungunya virus; EEEV, eastern equine encephalitis virus; ELISA, enzyme‐linked immunosorbent assay; PRNT, plaque reduction neutralization test; SLEV, St. Louis encephalitis virus; VEEV, Venezuelan encephalitis virus; WEEV, western equine encephalitis virus; WNV, West Nile virus.
Although not mosquito‐transmitted, neutralizing antibodies for Rio Bravo virus were also assayed. Rio Bravo virus is a flavivirus associated with bats and, if present, can influence the interpretation of antibody responses to other flaviviruses.
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