Table 2.
Intra‐ and interassay variability in the D1466 real‐time RT‐PCR
| Viral copies | C t mean | Intra‐assay (repeatability) | C t mean | Interassay (reproducibility) | ||
|---|---|---|---|---|---|---|
| CV | SD | CV | SD | |||
| 8·2 × 106 | 17·70 | 0·69 | 0·12 | 17·76 | 2·39 | 0·42 |
| 6·1 × 105 | 21·06 | 0·10 | 0·02 | 21·32 | 3·55 | 0·75 |
| 3·7 × 105 | 24·91 | 0·04 | 0·01 | 24·87 | 1·27 | 0·31 |
| 3·5 × 104 | 28·15 | 0·36 | 0·10 | 28·03 | 1·12 | 0·31 |
| 5·1 × 103 | 30·87 | 0·16 | 0·05 | 30·85 | 1·24 | 0·38 |
| 1·5 × 103 | 33·44 | 0·53 | 0·18 | 32·60 | 2·93 | 0·95 |
| 5·6 × 101 | 37·03 | 0·17 | 0·06 | 37·59 | 0·99 | 0·37 |
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