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. 2013 Feb 4;61(5):679–693. doi: 10.1002/glia.22461

Figure 7.

Figure 7

Endocytic activity in IFITM3‐transfected COS7 cells. MOCK‐ or IFITM3‐transfected COS7 cells were incubated with (A) AF555‐Tf (50 µg/mL) for 10 min or (B) AF555‐EGF (50 ng/mL) for 15 min. Scale bar: 20 µm. (C) Quantitative analysis of AF555‐labeled probes uptake in COS7 cells transfected with IFITM3. Values are the means ± SEM of two independent experiments (AF555‐Tf: n = 20–83, AF555‐EGF: n = 83–139). AF555‐Tf (F (2,121) = 13.65, P < 0.001) and AF555‐EGF (F (2,267) = 86.26, P < 0.0001). *P < 0.05 versus control COS7 cells, # P < 0.05 versus MOCK‐transfected COS7 cells. (D) Time‐course of AF555‐Tf recycling in COS7 cells transfected with IFITM3. Values are the means ± SEM of two independent experiments (n = 50–82).