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. 2013 Feb 4;61(5):679–693. doi: 10.1002/glia.22461

Figure 8.

Figure 8

Effect of IFITM3 expression on endocytic activity in cultured astrocytes. (A) WT or ifitm3−/− astrocytes treated with polyI:C were incubated with AF555‐Tf for 10 min or AF555‐EGF for 15 min in the presence or absence of CPM. Cultured astrocytes were treated with CPM (Sigma‐Aldrich, 10 µg/mL) for 60 min and then incubated with AF555‐EGF. Scale bar: 100 µm. (B) Quantitative analysis of AF555‐Tf and AF555‐EGF uptake in wt and ifitm3−/− astrocytes treated with polyI:C. Values are the means ± SEM of 2–3 independent experiments (n = 40–120). AF555‐Tf: polyI:C (F (1,416) = 256.07, P < 0.0001), genotype (F (1,416) = 19.93, P < 0.0001), interaction (F (1,416) = 3.78, P = 0.053). AF555‐EGF: polyI:C (F (1,155) = 11.21, P < 0.01), genotype (F (1,155) = 52.72, P < 0.0001), interaction (F (1,155) = 14.8, P < 0.01). AF555‐EGF with CPM: polyI:C (F (1,205) = 6.85, P < 0.01), genotype (F (1,205) = 73.99, P < 0.0001), interaction (F (1,205) = 1.48, P = 0.23). *P < 0.05 versus vehicle‐treated wt control astrocytes, # P < 0.05 versus polyI:C‐treated wt astrocytes, + P < 0.05 versus vehicle‐treated ifitm3−/− astrocytes.