Table 1.
Primers and Probes Used in This Study
Name | Origin | Assay, Orientation | Sequence (5′–3′) | Coordinates* |
---|---|---|---|---|
Primer C | This study | Real‐time PCR, antisense | AGT TGC ATG ACA GCC CTC TAC A | 18260‐18245 |
Primer D | This study | Real‐time PCR, sense | CCC GCG AAG AAG CTA TTC G | 18193‐18211 |
Probe E | This study | Real‐time PCR, flurogenic probe | CGT TCG TGC GTG GAT TGG CTT TG | 18215‐18237 |
SAR1S | CDC† | Conventional PCR, sense | CCT CTC TTG TTC TTG CTC GCA | 15291‐15271 |
SAR1As | CDC† | Conventional PCR, antisense | TAT AGT GAG CCG CCA CAC ATG | 15371‐15391 |
COR1 | GVU‡ | Conventional PCR, sense | CAC CGT TTC TAC AGG TTA GCT AAC GA | 15318‐15343 |
COR2 | GVU‡ | Conventional PCR, antisense | AAA TGT TTA CGC AGG TAA GCG TAA AA | 15628‐15603 |
BNIoutS2 | BNI§ | Conventional PCR, sense | ATG AAT TAC CAA GTC AAT GGT TAC | 18153‐18176 |
BNIoutAs | BNI§ | Conventional PCR, antisense | CAT AAC CAG TCG GTA CAG CTA C | 18342‐18321 |
Tor2 (GenBank # AY274119).
Centers for Disease Control and Prevention, Atlanta, GA, USA.
Government Virus Unit, Hong Kong SAR, China.
Bernard‐Nocht Institute for Tropical Medicine, Hamburg, Germany.