Table 2.
Immunogenic proteins reported using the viral polypeptide expression system in plants
| Polypeptide expression system | Expressed sequence | Plant used for infection | Species protected | Immunological properties | Reference |
|---|---|---|---|---|---|
| TMV | VP1 from the foot and mouth disease virus (FMDV) | Nicotiana benthamiana | Mouse | Parenteral application in the presence of CFA protected all animals in two separate experiments | 47 |
| TMV | 38C13 scFv specific to the 38C13 mouse B‐cell lymphoma | Nicotiana benthamiana | Mouse | Mice vaccinated with the affinity purified 38C13 scFv generated >10 µg/mL anti‐idiotype immunoglobulins; these mice were protected from challenge by a lethal dose of the syngeneic 38C13 tumour | 43 |
| TMV | Betv1, a major birch pollen antigen | Nicotiana benthamiana | Mouse | Parenteral application with crude leaf extracts generated immunological responses comparable to those induced by the protein expressed in Escherichia coli or extracted from birch pollen | 48 |
| TMV | gDc from bovine herpes virus type 1 (BHV‐1) | Nicotiana benthamiana | Mouse Cattle | Parenteral application of oil‐based vaccines with crude extracts protected both animals | 49 |
| PVX | E7 from human papillomavirus 16 (HPV‐16) | Nicotiana benthamiana | Mouse | Parenteral application with foliar extracts in the presence of Quil A as an adjuvant developed both antibody and cell‐mediated immune responses | 54 |
| PPV | VP60 from rabbit haemorrhagic disease virus (RHDV) | Nicotiana clevelandii | Rabbit | Parenteral application with crude extracts in the presence of adjuvant fully protected rabbits against subsequent challenge with a lethal dose of RHDV | 57 |
| CPMV | Small immunogenic proteins (∈SIP) specific to transmissible gastroenteritis virus (TGEV) | Vigna unguiculata | Pig | Oral application using ∈SIP infected crude leaf powder | Lomonossoff (unpubl. data, 2005) |
CPMV, cowpea mosaic virus; PPV, plum pox virus; PVX, potato virus X; TMV, tobacco mosaic virus.