Figure 3.

Demonstration of the importance of inflammation on the phenotype of misfolding disease. Production of goblet cell mucins stained with Alcian blue and PAS in mice with a Muc2 mucin misfolding mutation (Winnie) and in Winnie mice treated with the anti‐inflammatory drug 6‐thioguanine (6‐TG). Goblet cells in Winnie mice have small blue‐staining thecae (a small reservoir of granules of Muc2 for secretion) and large accumulations of pink‐staining misfolded protein. After treatment with 6‐TG, mucin production is restored (large blue thecae of stored mucin granules), although a small amount of misfolded protein is still seen. A full colour version of this figure is available at the Immunology and Cell Biology journal online.