Figure 7.

CTL induction from PBMCs of HLA‐A2‐positive healthy donors. FOXM1 peptide‐reactive CTLs were generated from CD8⁺ T cells of HLA‐A2⁺ healthy donors. After 3 or 4 times of stimuli with autologous monocyte‐derived DCs pulsed with the FOXM1‐8, FOXM1‐10, or FOXM1‐17 peptide, the CTLs were subjected to IFN‐γ ELISPOT assay, using T2 cells pulsed with the same FOXM1 peptide as stimulators. The number of specific spots was determined by subtracting the mean number of spots in the absence of an antigen from that in the presence of the antigen: (ELISPOT number stimulated with T2 cells + peptide) − (ELISPOT number stimulated with T2 cells alone).