Figure 8.

Natural processing of FOXM1 peptides. (a) Total cell lysate from Caco‐2 cells infected with Lentiviruses without FOXM1‐gene (Caco‐2‐mock) or those introduced with HA‐tagged human FOXM1‐gene (Caco‐2‐FOXM1) was analyzed by Western blot analysis for transgene‐derived FOXM1expression, using anti‐HA antibody. As a loading control, the same membrane was blotted with an anti‐β‐actin antibody. (b) Cytotoxic activity of the CTL lines established from HLA‐A*0201‐positive healthy donors and patients with cancer by stimulation with the 3 FOXM1‐derived peptides against Caco‐2‐mock and Caco‐2‐FOXM1 (FOXM1⁺, HLA‐A*0201 ⁺) was examined by ⁵¹Cr release assay. The percentage of specific lysis was calculated from the mean values of duplicate or triplicate assays.