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. 2020 Apr 15;8:e8909. doi: 10.7717/peerj.8909

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©2020 Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A, B) Alizarin Red S Staining was performing on day 28 of osteoblast differentiation. (C) To quantify the amount of alizarin red staining in different groups, ^∗∗∗p < 0.001. (D) Quantitative evaluation of the osteogenic differentiation capacity using alkaline phosphatase (ALP) activity assay, ^∗∗∗p < 0.001. (E) The expression levels of RUNX2, Osx and ALP mRNA were examined by qPCR, ^∗∗∗p < 0.001. (F) qPCR results also showed that the expression of LOC100506178 was significantly up-regulated in BMP2 stimulated hBMSCs, ^∗∗∗p < 0.001.