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. 2020 Apr 15;8:e8909. doi: 10.7717/peerj.8909

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©2020 Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Putative binding sites of miR-214-5p in LOC100506178 were shown. (B) miR-214-5p was increased in shLOC100506178 transfected hBMSCs and decreased in LOC100506178 overexpression plasmids transfected hBMSCs, ^∗∗p < 0.01. (C) Correlation analysis between LOC100506178 and miR-214-5p levels in hBMSCs at 0, 1, 3, 5, 7, 14 and 28 d after osteogenic differentiation. (D) Luciferase reporter assay showed the directly reaction between LOC100506178 and miR-214-5p. (E) RIP assay demonstrated that LOC100506178 and miR-214-5p expression levels were significantly higher in the anti-AGO1 group compared with the anti-normal IgG group.