Fig. 1.

A schematic showing the possible outcomes of the delivery of two independent amplicons containing gene expressing HAC vectors after co-transduction into human cells. One amplicon vector contains 17 alphoid DNA and RFP (red), and the other amplicon vector lacking alphoid DNA and GFP (green). After selection of positive cells (yellow), a functional HAC containing both input genes (yellow) is generated (GFP + RFP HAC). The other outcomes show integrations of either input vector DNA (red or green, independent integrations) into host chromosomes, or co-localized on the same chromosome (yellow, co-integration), or a HAC containing 17 alphoid DNA only (red, RFP HAC) and GFP integration into a host chromosome (green).