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. 2020 Apr 7;31(1):107490. doi: 10.1016/j.celrep.2020.03.054

Figure 2.

Figure 2

Divergent SHH Signal Transduction in Mouse and Pig Limb Buds

(A–F) Expression of Shh (A and B), Ptch1 (C and D), and Gli1 (E and F) in mouse and pig limb buds at equivalent stages of limb development.

(G) Expression ratio of Ptch1 and Gli1 in mouse (E11.25) and pig (D23) limb buds dissected into anterior and posterior halves using qRT-PCR analysis (data are represented as mean ± SD; n = 9 for mouse and n = 7 for pig limb buds). HL, hindlimb bud. Scale bars, 0.25 mm. ns, non-significant (p = 0.68); ∗∗p = 0.0073. n = 2 per stage for all pig ISH probes; n = 3 per stage for all mouse ISH probes.