Figure 5.
Evolutionarily Conserved Regions with Divergent Chromatin Accessibility Associated with Genes Essential for Limb Morphogenesis
(A) Limb regulatory genes (see Table S4) with associated mCCP and/or pCCM regions (assigned to the closest TSS).
(B) The list of 445 limb phenotype (LP) genes associated with regions of differential chromatin accessibility in the two species (see Table S5). This list encompasses genes functioning in the SHH, WNT, BMP/TGFβ, and FGF signaling pathways (see Table S6).
(C) Sulf1 expression in mouse (n = 3) and pig (n = 2) handplates. Scale bars, 0.25 mm. Right: HiC contact profile (Dixon et al., 2012) of the mouse Sulf1 TAD (top panel). Additional genes located in the TAD are indicated in gray. ATAC-seq profiles from mouse (E10.5/E11.5) and pig forelimb buds (D21/D24) (bottom panel).