Figure 5. Quantitative RT-PCR confirming the differential regulation of isoniazid responsive genes and genes indicative of bacterial metabolic state in log-phase and NRP2 relative to drug-free log-phase bacilli.
Expression ratios ± isoniazid (INH), measured in fold-change, were calculated relative to sigA and untreated log-phase control using the ΔΔCt method. Standard deviations from triplicate samples are marked.
AG: Aerobic; NRP: Nonreplicating persistence.