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Kinetics of inactivation of SmPutA by T2C. The points represent the PRODH activity measured after preincubating SmPutA with 2 mM T2C for various time periods and removing the excess T2C. The error bars represent the standard deviation of three technical replicates. The curve represents the fit to an exponential decay function, which yields an apparent pseudo-first order rate constant for enzyme inactivation of 0.44 ± 0.08 days−1.
