Figure 5. Reactome analysis of overlapping gene hits from 29i and 30i ALS/FTD C9+ cell lines.
Panel a, Reactome (https://reactome.org) computational analysis of the common gene hits from the qRT-PCR microarrays highlight the potential involvement of cellular senescence pathways in the CCR of the 29i and 30i cell lines after 12 weeks in 3D culture. Panel b, qPCR was performed on RNA from 29i, 30i and 7545 neurotypic control cells to quantify CXCL8 gene expression. Panel c, Luminex analysis was performed for detection of secreted CXCL8. N=3 replicates ± SEM. Error bars=SEM.