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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2020 Jan 22;199:105603. doi: 10.1016/j.jsbmb.2020.105603

Figure 1. Effect of dietary supplementation on body weight and vitamin D metabolites.

Figure 1.

(A) Study design, cohorts and end points. C57BL/6 mice were randomized to receive one of 3 vitamin D3 diets: 25 IU, 100 IU, or 10,000 IU. Six weeks after initiation of diets, mice were exposed to carcinogen 4NQO in drinking water for 16 weeks. Mice were fed regular drinking water for an additional 10 weeks. Mice were maintained on the assigned diets for the entire study. Blood was collected at week 0 (6 weeks post initiation of diet prior to 4NQO exposure) and at week 26 for determination of vitamin D3 metabolites by LC-MS/MS. At study termination (week 26), tongue tissues were collected for histology and immunohistochemistry. (B) Body weights of mice measured once every three days throughout the duration of the study did not reveal any significant difference between the three dietary regimens. Absolute levels of 25(OH)D3 (C) at week 26 and change in 25(OH)D3 levels of mice from week 0 to 26 (D) for three dietary vitamin D3 cohorts. Levels of 1,25(OH)2D3 (E) and calcium (F) at week 26 in animals maintained on the three diets.