Figure 6:

Prenatal ethanol exposure (2 g/kg/day, E10-E15) compared with Control and Untreated groups affected the single-labeled CXCR4⁺ and BLBP⁺ cells in the NEP of E19 embryos (n = 7/sex/group). A, Ethanol compared with control groups significantly increased the density of CXCR4⁺ (top) and BLBP⁺ (bottom) cells in both female and male embryos, with the effects in females significantly greater. B, This effect in E19 embryos is illustrated in representative immunostaining confocal images of single-labeled CXCR4⁺ (green) and BLBP⁺ (red) single-labeled neurons. Abbreviations: CXCR4, C-X-C Receptor 4; BLBP, brain lipid binding protein; E19, embryonic day 19; NEP, neuroepithelium; V, ventricle. Scale bar, 100 μm. Data are mean ± SEM. *p < 0.05 versus control group. #p < 0.05 versus male group.