Table 2:
Antibodies used for single-labeling immunofluorescence histochemistry
| Primary antibody | Dilution | Vendor | Secondary antibody | Dilution | Vendor |
|---|---|---|---|---|---|
| Goat anti-CXCR4 | 1:100 | Abcam, MA | Bio-Horse anti-Goat IgG + TSA | 1:100 | Vector, CA |
| Rabbit anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch |
| Laboratories Inc, PA | |||||
| Rabbit anti-MCH | 1:1000 | Phoenix | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch |
| Pharmaceuticals, CA | Laboratories. Inc, PA | ||||
| Mouse anti-NeuN | 1:100 | EMD Millipore, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch |
| Laboratories. Inc, PA | |||||
| Rabbit anti- | 1:500 | Phoenix | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch |
| Hcrt/OX | Pharmaceuticals, CA | Laboratories. Inc, PA |
Abbreviations: CXCR4, C-X-C receptor type 4; BLBP, brain lipid-binding protein; MCH, melanin-concentrating hormone; NeuN, neuronal nuclei; Hcrt/OX, hypocretin/orexin.