Table 3:
Antibodies used for double-labeling immunofluorescence histochemistry
| Combination | Primary antibody | Dilution | Vendor | Secondary antibody | Dilution | Vendor |
|---|---|---|---|---|---|---|
| CXCR4+BLBP | Goat anti-CXCR4 | 1:200 | Abcam, MA | Bio-Horse anti-Goat IgG + TSA | 1:100 | Vector, CA |
| Rabbit anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch | |
| Laboratories Inc, PA | ||||||
| CXCR4+NeuN | Goat anti-CXCR4 | 1:200 | Abcam, MA | Bio-Horse anti-Goat IgG + TSA | 1:100 | Vector, CA |
| Mouse anti-NeuN | 1:100 | EMD Millipore, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch | |
| Laboratories Inc, PA | ||||||
| CXCR4+Hcrt/OX | Goat anti-CXCR4 | 1:200 | Abcam, MA | Bio-Horse anti-Goat IgG + TSA | 1:100 | Vector, CA |
| Rabbit anti-Hcrt/OX | 1:500 | Phoenix Pharmaceuticals, CA | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch Laboratories Inc, PA |
|
| CXCR4+MCH | Goat anti-CXCR4 | 1:200 | Abcam, MA | Bio-Horse anti-Goat IgG + TSA | 1:100 | Vector, CA |
| Rabbit anti-MCH | 1:1000 | Phoenix Pharmaceuticals, CA | Cy3-Donkey anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch Laboratories Inc, PA |
Abbreviations: CXCR4, C-X-C receptor type 4; BLBP, brain lipid-binding protein; NeuN, neuronal nuclei; Hcrt/OX, hypocretin/orexin; MCH, melanin-concentrating hormone.