Table 2.
Experimental design of experiment 2. Mature denuded oocytes or cumulus oocyte complexes (COC) were previously incubated with a Ca2+ sensitive fluorescence probe (Fluro4-AM) for 40 min. Then after the identification of the basal [Ca2+]i during 3–6 min using inverted fluorescence microscope they were subjected to different experimental conditions as described below and [Ca2+]i levels registered
| Trial | Cell type (n) | Group | Treatment |
|---|---|---|---|
| 1 | Oocytes (7), COC (4) | UTP | 100 μM of UTP |
| 1 | Oocytes (8), COC (9) | Suramin100 | 100 μM of suramin |
| 1 | Oocytes (4), COC (9) | Suramin300 | 300 μM of suramin |
| 1 | Oocytes (3), COC (4) | Suramin100 + UTP | 100 μM of suramin and after 3 min to 100 μM of UTP |
| 1 | Oocytes (3), COC (4) | Suramin300 + UTP | 300 μM of suramin and after 3 min to 100 μM of UTP |
| 1 | Oocytes (3), COC (5) | CPA | 7.5 EG% + 7.5% DMSO |
| 2 | COC (5) | CPA + CPA | CPA (7.5% EG + 7.5% de DMSO) and after 5 min another CPA (15% EG + 15% DMSO) exposure (30–45 s) |
| 2 | COC (5) | Suramin100 + CPA + CPA | 100 μM of suramin and after 3 min CPA (7.5% EG + 7.5% DMSO) and then after 5 min another CPA (15% EG + 15% DMSO) exposure (30–45 s) |
| 2 | COC (5) | Suramin300 + CPA + CPA | 100 μM of suramin and after 3 min CPA (7.5% EG + 7.5% DMSO) and then after 5 min another CPA (15% EG + 15% DMSO) exposure (30–45 s) |