Table 4.
JNJ-47965567 does not alter proportions of DC subsets in SOD1G93A mice. CNS draining and non-draining lymph nodes from end stage β-CD- and JNJ-47965567-treated SOD1G93A mice were labelled with fluorochrome-conjugated mAb and the percentages of DC subsets, as well as relative expression of CD80 (MFI), assessed by flow cytometry. Results are mean ± SEM
| Cell typea | Draining lymph node | Non-draining lymph node | ||
|---|---|---|---|---|
| Vehicle (n = 13) | JNJ-47965567 (n = 17) | Vehicle (n = 10) | JNJ-47965567 (n = 18) | |
| % cDC (CD11chi MHCIIint) of total cells | 0.2 ± 0.3 | 0.3 ± 0 | 0.8 ± 0.2b | 0.9 ± 0.1b |
| CD80 MFI | 30.5 ± 6.7 | 44.5 ± 9.7 | 20.5 ± 3.8 | 41.5 ± 8.4 |
| % CD11b+ | 39.4 ± 3.7 | 39.3 ± 3.7 | 24.3 ± 3.1 | 30.0 ± 2.1 |
| CD80 MFI | 57.3 ± 8.5 | 82.7 ± 14.8 | 75.4 ± 18.1 | 107.2 ± 27.6 |
| % CD11b− | 57.2 ± 3.8 | 56.8 ± 3.8 | 75.7 ± 3.1 | 70.0 ± 2.1 |
| CD80 MFI | 17.4 ± 2.6 | 24.3 ± 3.3 | 13.8 ± 2.8 | 28.2 ± 5.6 |
| % mDC (CD11cint MHCIIhi) of total cells | 0.9 ± 0.2 | 0.7 ± 0.1 | 1.0 ± 0.4 | 0.8 ± 0.1 |
| CD80 MFI | 4.2 ± 1.8 | 10.6 ± 4.6 | 3.2 ± 0.7 | 13.3 ± 6.2 |
| % CD11b+ | 37.8 ± 1.6 | 39.5 ± 1.7 | 29.4 ± 3.9 | 28.6 ± 2.5 |
| CD80 MFI | 6.4 ± 3.6 | 14.6 ± 6.2 | 6.5 ± 2.3 | 19.4 ± 7.6 |
| % CD11b− | 57.9 ± 1.8 | 57.0 ± 1.8 | 70.6 ± 3.9 | 71.4 ± 2.5 |
| CD80 MFI | 3.2 ± 0.9 | 8.6 ± 4.1 | 2.7 ± 1.7 | 11.5 ± 5.9 |
acDC conventional DCs, mDC migratory DCs, MHCII MHC class II
bP < 0.05 compared to corresponding conventional DCs in draining lymph nodes