Figure 2.

Structure of shRNA‐expression constructs and shRNA sequences. (a) Structure of shRNA‐expression cassette and shRNA sequences. TT‐Loop, the loop sequence. The shRNA‐Control was directed toward an unrelated target, Machado–Joseph disease gene. Underlined letters indicate C‐to‐T point mutations in the sense strand. (b) The shRNA‐expression plasmids were transfected into Huh7/pRep‐Feo cells, and internal luciferase activities were measured at 48 h of transfection. Each assay was done in triplicate, and the values are displayed as mean + SD. *P < 0.05. (c) pLNCshRNA, structure of a recombinant retrovirus expressing shRNA. Ψ, the retroviral packaging signal sequence. AxshRNA, structure of a recombinant adenovirus expressing shRNA.