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. 2011 Jan 25;112(2):559–570. doi: 10.1002/jcb.22946

Figure 2.

Figure 2

EV71 infection induces the phosphorylation of CREB via c‐Src/EGFR. (A) Cells were pretreated with AG1478 for 1 h, followed by stimulation with EV71 for 30 min. (B) Cells were incubated with EV71 for the indicated time. (C) Cells were pretreated with AG1478 for 1 h prior to stimulation with EV71 for 5 min. (D) Cells were pretreated with PP1 for 1 h, followed by stimulation with EV71 for 5 (for p‐EGFR) or 30 (for p‐CREB) min. (E) Cells were treated with EV71 for the indicated time. (F) Cells were pretreated with AG1478 (10 µM) or PP1 (3 µM) for 1 h before treatment with EV71 for 5 min. The cell lysates were subjected to Western blot analysis using an anti‐CREB, anti‐p‐CREB (A, D), anti‐EGFR, and anti‐p‐EGFR (B, C, D) antibody. The cell lysates were subjected to immunoprecipitation using an anti‐c‐Src antibody and the immunoprecipitates were then analyzed by Western blot analysis using an anti‐c‐Src (E, F), anti‐c‐p‐Src (E), and anti‐p‐EGFR (E, F) antibody. Data are expressed as mean ± SEM of at least three independent experiments. *P < 0.05; # P < 0.01 as compared with the cells exposed to EV71 alone (A, C, D, F). *P < 0.05 as compared with the basal level (B, E).