Figure 5.

CREB associates with p300 and the resulting complex binds the COX‐2 promoter in SK‐N‐SH cells infected with EV71. (A) Cells were pretreated with GR343 for 1 h before incubation with EV71 for 12 h. (B) Cells were transfected with p300 siRNA, and were incubated with EV71 for 12 h. (C) Cells were incubated with EV71 for the indicated time. The cell lysates were subjected to immunoprecipitation using an anti‐p300 antibody. (D) Cells were incubated with EV71 for the indicated time and ChIP assays were then performed. (E) Cells were pretreated with H89 for 1 h, followed by incubation with EV71 for 30 min. The cell lysates were subjected to Western blot analysis using an anti‐COX‐2 (A, B), anti‐p300 (B) antibody. The immunoprecipitates were then analyzed by Western blot analysis using an anti‐p300 or anti‐CREB antibody (C). Chromatin was immunoprecipitated with anti‐CREB or anti‐p300 antibody and 1% of the precipitated chromatin was assayed to verify equal loading (“Input”) (D, F). Data are expressed as mean ± SEM of at least three independent experiments. *P < 0.05; ^# P < 0.01 as compared with the cells exposed to EV71 alone (A, B, E). *P < 0.05; ^# P < 0.01 as compared with the basal level (C, D).