Figure 6.

PGE₂ promotes the replication of EV71 through cAMP. (A) SK‐N‐SH cells first were infected with EV71 for 1 h. The infected cells were treated with H89 for 1 h before incubation with either PGE₂ or forskolin for 4 h. (B) Cells were infected with EV71 for 1 h and were treated with AH6809 or GW627368x as indicated, prior to incubation with PGE₂ for 4 h. The expression of VP1 mRNA was determined by real‐time PCR. Data are expressed as mean ± SEM of three independent experiments. ^# P < 0.01 for significant difference between the groups.