Table II.

Primers and Probes Used for the One‐Step Real‐Time Triplex RT‐PCR

Target	Amplicon size (bp)	Primers and probes	5′–3′ Nucleotide sequences (final concentration in nmol/L)
Influenza A segment 7	85	Forward	AGGCTCTCATGGAA/GTGGCTAAAGa (300)
		Reverse	ACGGTGAGCGTGAACACAAAa (300)
		Probe	VIC‐TGTCACCTCTGACTAAGG‐MGBa (100)
Influenza B segment 7	65	Forward	TGTCGCTGTTTGGAGACACAA (100)
		Reverse	TGCTTTGCCTTCTCCATCTTC (100)
		Probe	NED‐TGCCTACCTGCTTTCA‐MGB (200)
RSV‐A/B L gene	149	Forward	GTGGAACTTCATCCTGACATAAGATATATT (900)
		Reverse	GTTGCATCTGTAGCAGGAATGGT (900)
		Probe	FAM‐ATTGCAATGATCATAGTTTACCT‐MGB (200)

^aModified from Kuypers et al. [2006] and van de Pol et al. [2007].