Table III.
Ranking of Genes by Expression Stability M Calculated by geNorm (Stable Genes Have Lower M Values) and by Stability Value as Analyzed by NormFinder (the Lower the Stability Value Better the Reference Gene)
| moi 1 | moi 10 | moi 1 and 10 | |||
|---|---|---|---|---|---|
| geNorm (M‐value) | NormFinder (stability value) | geNorm (M‐value) | NormFinder (stability value) | geNorm (M‐value) | NormFinder (stability value) |
| Selected reference genes ranked in order of decreasing expression stabilitya | |||||
| HUEL/GAPDH (0.205) | GAPDH (0.011) | HUEL/HPRTI (0.204) | GAPDH (0.011) | HUEL/GAPDH (0.203) | GAPDH (0.011) |
| HUEL (0.040) | HUEL (0.039) | HUEL (0.039) | |||
| HPRTI (0.226) | ECHS (0.053) | GAPDH (0.220) | ECHS (0.058) | HPRTI (0.219) | ECHS (0.055) |
| TBP (0.331) | TBP (0.068) | ECHS (0.312) | HPRT1 (0.061) | ECHS (0.316) | HPRT1 (0.069) |
| ECHS (0.390) | HPRT1 (0.077) | TBP (0.423) | TBP (0.070) | TBP (0.402) | TBP (0.069) |
| RPII (0.553) | β‐actin (0.104) | β‐actin (0.575) | β‐actin (0.117) | β‐actin (0.559) | β‐actin (0.110) |
| β‐actin (0.621) | RPII (0.106) | RPII (0.642) | RPII (0.118) | RPII (0.559) | RPII (0.112) |
| B2MG (0.699) | PPIA (0.152) | B2MG (0.706) | PPIA (0.137) | B2MG (0.697) | PPIA (0.145) |
| PPIA (0.893) | B2MG (0.209) | PPIA (0.959) | B2MG (0.193) | PPIA (0.915) | B2MG (0.201) |
a
Data are given for paired cell samples (mock‐ and RV‐infected, n = 24). The two most stable reference genes identified by geNorm can not be grouped any further as geNorm algorithm is intended to identify a pair of most stable reference genes.