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. 2018 Sep 27;125(6):1670–1681. doi: 10.1111/jam.14073

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© 2018 The Society for Applied Microbiology

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Confocal laser scanning microscopic detection of surface expression of VP2 protein. Our results illustrate that there is a green fluorescent signal on the cell surface of strains pPG612‐VP2/LP, pPG612‐T7g10‐VP2/LP and positive control but no signals on negative control (pPG612/LP), indicating that the protein of interest is expressed and displayed successfully on the surface of recombinant Lactobacillus. a, b, c: pPG612/LP (negative control); d, e, f: pPG612‐VP2/Lactobacillus casei 393 (positive control); g, h, i: pPG612‐VP2/LP; j, k, l: pPG612‐T7g10‐VP2/LP. [Colour figure can be viewed at http://wileyonlinelibrary.com]