Table I.
Primer‐Probe Sequences Along With Final Concentrations in Nanomoles Used in Multiplex PCR Reaction
| Virus | Forward primer (5′–3′) [final concentration] | Reverse primer (5′–3′) [final concentration] | Taqman probe (5′–3′) –BHQ [final concentration] |
|---|---|---|---|
| Panel 1 | |||
| Flu A | CAT GGA RTG GCT AAA GAC AAG ACC [650 nM] | AGG GCA TTT TGG ACA AAK CGT CTA [650 nM] | TGC AGT CCT CGC TCA CTG GGC ACG [650 nM] |
| Flu B | TCC TCA ACT CAC TCT TCG AGC G [250 nM] | CGG TGC TCT TGA CCA AAT TGG [250 nM] | CCA ATT CGA GCA GCT GAA ACT GCG GTG [250 nM] |
| RSV | CACWGAAGATGCWAATCATAAATTCA [650 nM] | GTATYTTTATRGTGTCTTCYCTTCCTAACC [650 nM] | TAATAGGTATGTTATATGCKATGTC [650 nM] |
| Panel 2 | |||
| PIV‐1 | ACAGATGAAATTTTCAAGTGCTACTTTAGT [650 nM] | GCCTCTTTTAATGCCATATTATCATTAGA [650 nM] | ATGGTAATAAATCGACTCGCT [650 nM] |
| PIV‐2 | CCATTTACCTAAGTGATGGAA [250 nM] | CGTGGCATAATCTTCTTTTT [250 nM] | AATCGCAAAAGCTGTTCAGTCAC [250 nM] |
| PIV‐3 | TTA CARATAGGGA TAATAACTGT [650 nM] | TTAGGAGTGCTAGAGAACAT [650 nM] | AAACTCAGAC TTGGTACCTG ACTTAAAT [650 nM] |
| Panel 3 | |||
| hCoV 229E | CAGTCAAATGGGCTGATGCA [250 nM] | AAAGGGCTATAAAGAGAATAAGGTATTCT [250 nM] | CCCTGACGACCACGTTGTGGTTCA [250 nM] |
| hCoV OC43 | CGATGAGGCTATTCCGACTAGGT [250 nM] | CCTTCCTGAGCCTTCAATATAGTAACC [250 nM] | TCCGCCTGGCACGGTACTCCCT [250 nM] |
| Panel 4 | |||
| hMPV | CATCAGGTAATATCCCACAAAATCAG [500 nM] | GTGAATATTAAGGCACCTACACATAATAARA [500 nM] | TCAGCACCAGACACAC [500 nM] |
| EAV | GGCGACAGCCTACAAGCTACA [150 nM] | CGGCATCTGCAGTGAGTGA [150 nM] | TTGCGGACCCGCATCTGACCAA [150 nM] |
| Panel 5 | |||
| AdV | GGACGCCTCGGAGTACCTGAG [250 nM] | AC/ideoxyI/GTGGGGTTTCTGAACTTGTT [250 nM] | CTGGTGCAGTTCGCCCGTGCCA [250 nM] |
| hBoV | TGC AGA CAA CGC YTA GTT GTT T [200 nM] | CTG TCC CGC CCA AGA TAC A [200 nM] | CCA GGA TTG GGT GGA ACC TGC AAA [200 nM] |
| Panel 6 | |||
| CMV | CGA TCA AGA ACG CGA TAA CG [250 nM] | ACC GTC GAT GGC AGG TCA T [250 nM] | CGA TCA CAA ACA GCG [250 nM] |
| HHV‐6 | CGC TAG GTT GAG GAT GAT CGA [200 nM] | CAA AGC CAA ATT ATC CAG AGC G [200 nM] | CAC CAG ACG KCA CAC CCG AAG GAA T [200 nM] |
| HSV‐1 | CATCACCGACCCGGAGAGGGAC [250 nM] | GGGCCAGGCGCTTGTTGGTGTA [250 nM] | CCGCCGAACTGAGCAGACACCCGCGC [250 nM] |
| Flu A‐H1 | AGG CAA ATG GAA ATC TAA TAG CGC [200 nM] | CCA TTG GTG CAT TTG AGG TGA TG [200 nM] | TGA YCC AAA GCC “T”CT ACT CAG TGC GAA AGC [200 nM] |
| Flu A‐H3 | AAG CAT TCC YAA TGA CAA ACC [200 nM] | ATT GCR CCR AAT ATG CCT CTA GT [200 nM] | CAG GAT CAC ATA TGG GSC CTG TCC CAG [200 nM] |
| Flu A‐N1 | ATGGTAATGGTGTTTGGATAGGAAG [200 nM] | AATGCTGCTCCCACTAGTCCAG [200 nM] | TGATTTGGGATCCTAATGGATGGACAG [200 nM] |
| Flu A‐N2 | AAGCATGGCTGCATGTTTGTG [200 nM] | ACCAGGATATCGAGGATAACAGGA [200 nM] | TGCTGAGCACTTCCTGACAATGGGCT [200 nM] |
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