Figure 4.

 Effect of telbivudine treatment on the total number of T cells and the percentage of IFN‐γ and TNF‐α in CD4⁺ and CD8⁺ T cells. Mice were sacrificed 15 days after infection, and single‐cell suspensions were prepared from their spleen. Cells were stained with anti‐CD3, anti‐CD4 or anti‐CD8, and IFN‐γ or TNF‐α. Telbivudine treatment increased the total number of T cells, which was determined by the percentage of CD3⁺ cells in counted splenocytes (a). Telbivudine increased the percentage of IFN‐γ‐ and TNF‐α‐producing CD4⁺ T cells (b,d,f,g) and the percentage of IFN‐γ‐producing CD8⁺ T cells (c,f,g). The numbers in plots were assessed on CD4⁺ or CD8⁺ T cells. The results are a combination of three independent experiments. (b–e) are representative of three similar experiments. *P < 0.01.