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. 2010 Dec 22;83(2):291–297. doi: 10.1002/jmv.21956

Figure 2.

Figure 2

Phylogenetic analysis of human coronavirus based on the partial sequence of the gene ORF 1ab. Phylogenetic trees were constructed based on nucleotide sequences of PCR products corresponding to the partial pol gene of OC43, 229E, NL63, and HKU1, analyzed by MEGA4.0 software using the distance method and the neighbor‐joining algorithm with Kimura 2 parameters. Each strain from this study is indicated by a specific identification code (PUMCH) followed by the patient number and GenBank accession number. The GenBank accession numbers of the reference sequences of OC43, 229E, NL63, HKU1A and B were NC005147, NC002645, NC005831, NC006577, and AY884001, respectively. Scale bar indicates nucleotide substitutions per site.