Table I.
Performance Comparison of Individual Targets in Each Test
| Real time RT–PCR | Multiplex RT–PCR | Luminex xTAG | |||||
|---|---|---|---|---|---|---|---|
| Virus | No. true positive specimens | No. detected | % Sensitivity (95% CI) | No. detected | % Sensitivity (95% CI) | No. detected | % Sensitivity (95% CI) |
| Rhinovirus/Enterovirus | 86 | 57* | 66.2 (55.2, 76.1) | 75 | 87.2 (78.2, 93.4) | 75 | 87.2 (78.2, 93.4) |
| RSV‐B | 38 | 37 | 97.3 (86.1, 99.5) | 37 | 97.3 (86.1, 99.5) | 18 | 47.3 (30.9, 64.1) |
| H1N1pdm09 a | 35 | 35 | 100 (89.9, 100) | 35 | 100 (89.9, 100) | 19 | 54.2 (36.6, 71.1) |
| PIV‐3 | 18 | 17 | 94.44 (72.6, 99) | 18 | 100 (81.3, 100) | 8 | 44.4 (21.5, 69.2) |
| RSV‐A | 15 | 15 | 100 (78, 100) | 15 | 100 (78, 100) | 2 | 13.3 (2, 40.4) |
| HMPV | 7 | 5 | 71.4 (29.2, 95.4) | 7 | 100 (58.9, 100) | 6 | 85.7 (42.2, 97.6) |
| Influenza B | 5 | 5 | 100 (47.9, 100) | 5 | 100 (47.9, 100) | 1 | 20 (3.3, 71.1) |
| Corona OC43 | 6 | 6 | 100 (54, 100) | 4 | 66.6 (22.6, 94.6) | 6 | 100 (54, 100) |
| PIV‐4 | 9 | 4 | 44.4 (13.9, 78.6) | 9 | 100 (66.2, 100) | 7 | 77.7 (40, 96.5) |
| PIV‐1 | 2 | 2 | 100 (19.2, 100) | 2 | 100 (19.2, 100) | 1 | 50 (8.1 91.83) |
| Total sample positive | 199 | 175 | 193 | 138 | |||
*Real time RT‐PCR detect only Rhinovirus.
a
The xTAG® RVP fast kit detects influenza A only and do not have marker to subtype influenza A in H1N1pdm09.
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