Figure 2.

Schematic representation of SHERLOCK; In the presence of appropriate RPA primers, the target sequence that needs to be identified is subjected to amplification through the isothermal RPA or RT‐RPA. The amplified DNA sequence obtained that is subjected to in vitro T7‐transcription. The produced RNA molecule can be recognized via fluorescence signals that are generated by the reporter molecule upon target recognition by the Cas13 RNAse.4 The cleaved reporter molecule can also be detected in the form of bands through a lateral flow assay. The inclusion of Csm6 could improve the amplification of the output signal11