Table 3.
Effect of SjGCP s1 siRNA‐induced SjGCP inhibition on burden parasites and parasite pairing at 19, 28 and 32 days post‐infection in mice challenged with S. japonicum, followed by injection of siRNAs
| Group | Subgroup | Worm number | Pairing | |||
|---|---|---|---|---|---|---|
| Number of parasites (mean ± SD) | % Reduction | Number of pairs (mean ± SD) | % Pairing | % Inhibition | ||
| 19 days | Untreated (n = 7) | 37 ± 20 | 0 | 14 ± 6.8 | 76.8 ± 9.4 | 0 |
| Irrelevant (n = 7) | 34 ± 15 | 0 | 12 ± 4 | 74.3 ± 14.3 | 0 | |
| SjGCP s1 (n = 7) | 30 ± 6 | 27 | 5 ± 2 | 36.3 ± 11.4 | 52 p < 0.05 | |
| SjGCP cm s1 (n = 5) | 29 ± 5 | 20 | 3 ± 1 | 19.6 ± 5.3 | 74 p < 0.05 | |
| 28 days | Untreated (n = 8) | 46 ± 3 | 0 | 20 ± 5 | 89.4 ± 6.9 | 0 |
| Irrelevant (n = 8) | 63 ± 5 | 0 | 28 ± 5 | 88.9 ± 6.9 | 0 | |
| SjGCP cm s1 (n = 8) | 38 ± 6 | 35 p < 0.05 | 13 ± 3 | 55.47 ± 15.6 | 38 p < 0.05 | |
| 32 days | Untreated (n = 8) | 50 ± 7 | 0 | 24 ± 6 | 98.5 ± 3.3 | 0 |
| Irrelevant (n = 8) | 53 ± 11 | 0 | 26 ± 3 | 98.3 ± 2.4 | 0 | |
| SjGCP cm s1 (n = 8) | 34 ± 9 | 36 p < 0.05 | 16 ± 4 | 91.6 ± 7.7 | 7.2 p > 0.05 | |
n, number of mice per group; s1, unmodified s1 siRNA; cm s1, chemically modified s1 siRNA.
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