Table I.

Primer and Probe Sequences of HCoV‐HKU1 Used in Real‐Time PCR Assay

Gene	Primer/Probe	Sequence (5′–3′)	Position
ORF 1a	Forward	GTTGGTTGTATGATGCGTTTGTTCT	7,922–7,946
	Reverse	TCTACAAATAAACTAGCATCAACATCATCGT	7,971–8,001
	Probe	FAM‐CACGCTGTCCATCTCT‐NFQ	7,952–7,967
ORF 1b	Forward	CCTAACTGTGATGTGAGTGATGTCA	16,516–16,540
	Reverse	ACAAACCAAAGACCATACCATTCATAACT	16,608–16,636
	Probe	FAM‐CATACCGCCCAAATAT‐NFQ	16,548–16,563

FAM, 6‐carboxylfluorescein; NFQ, non‐fluorescent quenchers.