Table II.
Primer and Probe Sequences for Each of the 10 Targets Detected by the Real‐Time Respiratory RT‐PCR System
| Virus | Sequence | Final conc. (nM) | |
|---|---|---|---|
| Influenza A (FLA) | Forward | CAAGACCAATCCTGTCACCTCTG | 900 |
| Reverse | TGCATTTTGGACAAAGCGTCTAC | 900 | |
| Probe | FAM‐AGTCCTCGCTCACTGGGCACGGT‐BHQ1 | 200 | |
| Influenza B (FLB) | Forward | GGCAACACCTGTTTCCATATTAAG | 900 |
| Reverse | GCCTGTTAATGCAGACAAAACTC | 900 | |
| Probe | JOE‐TCATAGGCAGCTCCGAAGCAAGACATGA‐BHQ1 | 200 | |
| Respiratory syncytial virus (RSV) | Forward | GAAGGGTCAAACATCTGTTTAACAAG | 900 |
| Reverse 1 | GTTTCAGCTWGTGGGAAGAAAGATA | 900 | |
| Reverse 2 | GTGTCAGCCTGTGGAAAGAAGGATA | 900 | |
| Probe | FAM‐TGATCCTGCATTRTCACARTACCATCCTCT‐BHQ1 | 200 | |
| Parainfluenza 1 (PF1) | Forward | ATTCAGACAGGATGGAACCGTTAA | 900 |
| Reverse | GATACTAAGCTTTGTTGTGACCTCAT | 900 | |
| Probe | JOE‐ACCAATGCCTTCAACTGTGTCTCCCGTG‐BHQ1 | 200 | |
| Parainfluenza 2 (PF2) | Forward | AGAGATAACAGGGTTTGAGAATAATTCAT | 900 |
| Reverse | CAAATGGAGTTTGGTGATTAAGGGTA | 900 | |
| Probe | FAM‐TCCAGATGCTCGATCAACTATGTCCAGAGG‐BHQ1 | 200 | |
| Parainfluenza 3 (PF3) | Forward | CGATTAGAGGCTTTCAGACAAGATG | 900 |
| Reverse | CTGTTGAGACCGCATGATTGAC | 900 | |
| Probe | Cy5‐CCACTGTGTCACCGCTCAATACCAGCC‐BHQ3 | 200 | |
| Enterovirus | Forward | CCCCTGAATGCGGCTAATCC | 900 |
| Reverse | GTCACCATAAGCAGCCAATATAAGAA | 900 | |
| Probe | AACACGGACACCCAAAGTAGTCGGTTCC | 200 | |
| Adenovirus (ADV) | Forward | GCCCCAGTGGTCTTACATGCACATC | 900 |
| Reverse | GCCACGGTGGGGTTTCTAAACTT | 900 | |
| Probe | FAM‐TGCACCAGACCCGGGCTCAGGTACTCCGA‐BHQ1 | 200 | |
| Rhinovirus (HRV) | Forward 1 | TGTGCTCRCTTTGAGTCCTC | 900 |
| Forward 2 | TGTGCTCAGTGTGCTTCCTC | 900 | |
| Reverse | TGAAACACGGACACCCAAAGTA | 900 | |
| Probe | Cy5‐CCCTGAATGYGGCTAACCTTAAMCCTGC‐BHQ3 | 200 | |
| Metapneumovirus (MPNV) | Forward | CATATAAGCATGCTATATTAAAAGAGTCTC | 500 |
| Reverse | CCTATTTCTGCAGCATATTTGTAATCAG | 250 | |
| Probe | FAM‐TGYAATGATGAGGGTGTCACTGCGGTTG‐BHQ1 | 500 | |
| K‐Ras | Forward | GCCTGCTGAAAATGACTGAATATAAAC | 600 |
| Reverse | TGATTCTGAATTAGCTGTATCGTCAAG | 600 | |
| Probe | JOE‐TGCCTACGCCACAAGCTCCAACTACCA‐BHQ1 | 100 | |
| PDV | Forward | GCGGGTGCCTTTTACAAGAAC | 600 |
| Reverse | CAGAATAAGCAAAATTGATAGGAACCAT | 600 | |
| Probe | FAM‐TCTTTCCTCAACCTCGTCCGTCACAAGT‐BHQ1 | 100 | |